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Liver - New Organ Alliance

Liver
Cell Sourcing Challenges

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liver
sub-challenges

  • Improve understanding of the cultureability of hepatocytes and the limitations of producing hepatocytes in a culture. This requires new techniques to expand cells that do not currently expand.
  • Develop methods to isolate and identify tissue specific stem/progenitor cells.
  • Understand how to drive differentiation to hepatocytes, Cholangiocytes, and others.
  • Criteria for successful cell survival and expansion are required.
  • Media choice to keep all cells expanding/functioning though a heparinized blood analogue would be very useful.  Cells will need to be adapted to blood prior to implantation.

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